Test Code ADAMS ADAMTS13 Activity Assay, Plasma
Ordering Guidance
Consider ordering in patients with known diagnosis of congenital, immune, or acquired thrombotic thrombocytopenic purpura.
Specimen Required
Patient Preparation: Fasting preferred
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vials
Specimen Volume: 2 mL in 2 plastic vials each containing 1 mL
Collection Instructions:
1. Specimen must be collected prior to replacement therapy.
2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
4. Aliquot plasma (1 mL per aliquot) into 2 separate plastic vials, leaving 0.25 mL in the bottom of centrifuged vial.
5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, below -40° C.
Specimen Stability Information: Frozen 2 years
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
Useful For
Assisting with the diagnosis and monitoring of congenital, immune, or acquired thrombotic thrombocytopenic purpura
Special Instructions
Method Name
Fluorescence Resonance Energy Transfer (FRET)
Reporting Name
ADAMTS13 Activity Assay, PSpecimen Type
Plasma Na CitSpecimen Minimum Volume
2 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Plasma Na Cit | Frozen |
Reference Values
≥70%
Although not verified, the pediatric (<1 years old) reference range could be similar to or lower than that of adults.
Day(s) Performed
Monday through Friday, Sunday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
85397
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ADAMS | ADAMTS13 Activity Assay, P | 53622-7 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
620816 | ADAMTS13 Activity | 53622-7 |
620819 | Interpretation | 69049-5 |
Forms
If not ordering electronically, complete, print, and send one of the following with the specimen:
-Renal Diagnostics Test Request (T830)
-Coagulation Test Request (T753)