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Test Code LAB1134 Osmotic Fragility, Erythrocytes

Additional Codes

Mayo Test ID
FRAG

46235

Reporting Name

Osmotic Fragility, RBC
OhioHealth Laboratory Services Note:

Remember to draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

Useful For

Evaluating suspected hereditary spherocytosis-associated hemolytic anemia

 

Confirming or detecting mild spherocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility No Yes
SCTRL Shipping Control Vial No Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Control
Whole Blood EDTA


Ordering Guidance


 



Shipping Instructions


Specimens must arrive within 72 hours of collection.



Necessary Information


Patient's age is required.



Specimen Required


Both patient and shipping control whole blood specimens are required. For complete instructions, refer to Specimen Collection and Labeling Instructions for Osmotic Fragility Testing of Erythrocytes.

 

Shipping Control Specimen

Specimen Type: Whole blood (non-patient)

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a shipping control specimen from a healthy person unrelated to the patient at the same time (or within 4 hours) as the patient specimen.

Note: The shipping control specimen can be collected from a phlebotomist, volunteer or another heathy patient.

2. Clearly handwrite "CONTROL" on the outermost label of the tube.

3. Immediately refrigerate (or place on cold gel pack/small amount of wet ice) after collection.

4. Send shipping control in the original tube. Do not aliquot.

5. Keep the shipping control and the patient specimens together, either rubber banded or in a bag.

Additional Information:

1. The shipping control and patient specimens must be handled identically from the time of collection through receipt in the testing laboratory.

2. If the shipping control is not sent with the patient specimen, test cancellation is likely.

3. The shipping control specimen evaluates whether the patient result has been compromised by handling conditions such as temperature, motion, or other transportation interferences, as temperature and handling extremes can adversely impact the integrity of the specimen.

 

Patient Specimen

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect and label patient specimen.

2. Immediately refrigerate (or place on cold gel pack/small amount of wet ice) specimen after collection.

3. Send whole blood specimen in the original tube. Do not aliquot.

4. Keep the shipping control and the patient specimens together, either rubber banded or in a bag.

Additional Information: The shipping control and patient specimens must be handled identically from the time of collection through receipt in the testing laboratory.


Specimen Minimum Volume

Patient whole blood, shipping control: 2 mL each

Specimen Stability Information

Specimen Type Temperature Time
Control Refrigerated 72 hours PURPLE OR PINK TOP/EDTA
Whole Blood EDTA Refrigerated 72 hours

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

NaCl = Sodium chloride

Reference values have not been established for patients who are younger than 12 months

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85557

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRAG Osmotic Fragility, RBC 98904-6

 

Result ID Test Result Name Result LOINC Value
9064 Osmotic Fragility, RBC 34964-7
SCTRL Shipping Control Vial 40431-9
3306 Osmotic Fragility, 0.50 g/dL NaCl 23915-2
3307 Osmotic Fragility, 0.60 g/dL NaCl 23918-6
3308 Osmotic Fragility, 0.65 g/dL NaCl 23920-2
3309 Osmotic Fragility, 0.75 g/dL NaCl 23921-0
3310 Osmotic Fragility Comment 59466-3

Report Available

2 to 5 days

Method Name

Osmotic Lysis

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.