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Mayo Clinic Laboratories

Test Code LAB118 Protein, Total, Plasma or Serum

Reporting Title

Total Protein

Methodology

Roche Diagnostics, Biuret- RMH, GMC, DH, DMH, GMH, WECC, PMC, GCMH, BH, OBH, MGH, MH, HMH, SH

Protein levels are affected by a wide variety of liver, kidney, bone marrow, metabolic, and nutritional disorders.

 

VWH Lab Only:

TP reagent, when used in conjunction with UniCel® DxC 860i System(s) and SYNCHRON® Systems Multi Calibrator, is intended for the quantitative determination of Total Protein concentration in human serum or plasma.

Performing Laboratory

OhioHealth Laboratory Services-RMH,GMC,DH,DMH, MGH, GMH, HMH, WECC, PMC, OBH, MH, SH, GCMH, BH and VWH Core Laboratories

Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

 

Submit only 1 of the following specimens:

 

Preferred:

Serum

Draw blood in a gold-top gel tube(s). Spin down and send 1 mL of serum refrigerated.
Note:

1. A speckled-top serum gel tube(s) or plain red-top is also acceptable.  .

2. Indicate serum on request form.

3. Label specimen appropriately (serum).

 

Alternate (Preferred for VWH Lab): 

Plasma

Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated.

Note: 

1. Indicate plasma on request form.

2. Label specimen appropriately (plasma).

VWH Lab: 

3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells within two hours from the time of collection.

4. Separated serum or plasma should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, serum or plasma should be stored at +2°C to +8°C for up to 48 hrs. 

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Serum or plasma Refrigerated 1 month
  Ambient   
  Frozen  6 months

 

Specimen Transport Temperature

Refrigerate/Ambient OK/Frozen OK

Reference Values

0-6 days:  4.6-7.0 g/dL

7-8 days:  4.6-7.6 g/dL

9 days-7 months:  5.0-7.5 g/dL

8 months-1 year:  5.1-7.3 g/dL

2-3 years:  5.6-7.5 g/dL

>4 years:  6.0-8.0 g/dL

Note:  Protein, total is 0.4-0.8 g/dL lower when a patient is recumbent than it is when patient is upright.

 

VWH Lab Only: 

Adult Ambulatory: 6.4 – 8.3 g/dL

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

84155