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HelpTest Code LAB124 Testosterone, Plasma or Serum
Reporting Title
Testosterone
Methodology
Roche Diagnostics Electrochemiluminescence Immunoassay
Male testosterone is produced by testes, and is regulated by luteinizing hormone, subject to negative feedback from pituitary and hypothalamus. Female testosterone is produced by ovaries. Elevated levels (from ovarian tumor or hyperplasia or adrenal tumor hyperplasia) are associated with androgenic syndrome and polycystic ovaries.
Performing Laboratory
OhioHealth Laboratory Services-RMH Special Testing and MH Core Laboratory
Specimen Requirements
Due to interference, patients receiving high doses of biotin (>5 mg/day) should wait at least 8 hours after dose before having blood drawn.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable..
2. Indicate serum on request form.
3. Label specimen appropriately (serum).
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Alternate:
Plasma
Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated.
Note: 1. Indicate plasma on request form.
2. Label specimen appropriately (plasma).
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum and plasma | Refrigerated | 14 days |
| Ambient | 5 days | |
| Frozen | 6 months |
Specimen Transport Temperature
Refrigerate/Ambient OK/Frozen OK
Reference Values
Males
<1 year: 12-21 ng/dL
1-6 years: 3-32 ng/dL
7-12 years: 3-68 ng/dL
13-17 years: 28-1,110 ng/dL
18-50 years: 280-1,000 ng/dL
≥51 years: 280-800 ng/dL
Females: 6-82 ng/dL
Day(s) Test Set Up
RMH Special Testing:
Monday through Friday Continuous
Saturday and Sunday Day shift only
Test Classification and CPT Coding
84403