Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Submit only 1 of the following specimens:

Preferred:

Serum

Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated.    Specimen is usually drawn just prior to next oral dose. 

Note:

1.A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

2. Two weeks of therapy may be necessary to achieve steady-state.

3. Coadministration of barbiturates, benzodiazepines, phenytoin, succinimides, or valproic acid may induce lower serum levels but better seizure control. Patients may become toxic at lower levels. Carbamazepine may decrease levels of phenytoin and warfarin.
4. Date and time of draw is required on request form and specimen for processing.

5. Indicate serum on request form.

6. Label specimen appropriately (serum).

Alternate: Preferred Siemens Users

Plasma

Draw blood in a green-top (heparin) tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. Specimen is usually drawn just prior to next oral dose. 

Note:  1. Two weeks of therapy may be necessary to achieve steady-state.

2. Coadministration of barbiturates, benzodiazepines, phenytoin, succinimides, or valproic acid may induce lower serum levels but better seizure control. Patients may become toxic at lower levels. Carbamazepine may decrease levels of phenytoin and warfarin.
3. Date and time of draw is required on request form and specimen for processing.

4. Indicate plasma on request form.

5. Label specimen appropriately (plasma).

Specimen Stability Information