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Test Code LAB246 Culture, Blood, Mycobacterium

Reporting Title

Blood AFB Culture

Methodology

Culture

Automation (continous monitoring)

Isolation and identification of Acid-Fast Bacilli (AFB), Mycobacterial organisms

Susceptibility testing when appropriate

Performing Laboratory

OhioHealth Laboratory Services-RMH Microbiology

Specimen Requirements

Collect blood in BD Myco/ F Lytic blood culture bottle.

1. Vigorously cleanse venipuncture site using ChloraPrep®. Scrub in a back and forth motion for 30 seconds.

2. Allow site to dry. Do not touch site after preparation and prior to venipuncture.

3. Disinfect rubber top of blood culture bottle with alcohol and allow to dry.

4. Apply tourniquet, insert needle into vein, and withdraw 3-5 mL of blood. Do not change needles before injecting blood into the BD Myco/ F Lytic blood culture bottle.

5. Mix well to avoid clotting.

Note: Collection can be performed by needle and syringe or by using a butterfly with an adaptor for direct inoculation into tube.

6. Label tube with patient’s name (first and last), MR# or DOB, date and actual time of draw, and type of specimen.

7. Do not cover bottle barcode with patient label.

8. Maintain sterility and forward promptly at ambient temperature only.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Reference Values

No growth after 6 weeks

Critical value All positives

Alert category: Always called within 1 hour

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

87116 - culture, Mycobacterium

87118- culture, Mycobacterium ID