Test Code LAB246 Culture, Blood, Mycobacterium
Reporting Title
Blood AFB Culture
Methodology
Culture
Automation (continous monitoring)
Isolation and identification of Acid-Fast Bacilli (AFB), Mycobacterial organisms
Susceptibility testing when appropriate
Performing Laboratory
OhioHealth Laboratory Services-RMH Microbiology
Specimen Requirements
Collect blood in BD Myco/ F Lytic blood culture bottle.
1. Vigorously cleanse venipuncture site using ChloraPrep®. Scrub in a back and forth motion for 30 seconds.
2. Allow site to dry. Do not touch site after preparation and prior to venipuncture.
3. Disinfect rubber top of blood culture bottle with alcohol and allow to dry.
4. Apply tourniquet, insert needle into vein, and withdraw 3-5 mL of blood. Do not change needles before injecting blood into the BD Myco/ F Lytic blood culture bottle.
5. Mix well to avoid clotting.
Note: Collection can be performed by needle and syringe or by using a butterfly with an adaptor for direct inoculation into tube.
6. Label tube with patient’s name (first and last), MR# or DOB, date and actual time of draw, and type of specimen.
7. Do not cover bottle barcode with patient label.
8. Maintain sterility and forward promptly at ambient temperature only.
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Reference Values
No growth after 6 weeks
Critical value All positives
Alert category: Always called within 1 hour
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
87116 - culture, Mycobacterium
87118- culture, Mycobacterium ID