Test Code LAB26 Gentamicin, Trough/Pre-Dose, Plasma or Serum
Reporting Title
Gentamicin Pre-Dose
Methodology
Roche Diagnostics
Gentamicin has a narrow therapeutic index and an unpredictable dose-serum profile curve.
Performing Laboratory
OhioHealth Laboratory Services - RMH and MH Core Laboratories
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s) immediately prior to next dose (intravenous piggyback [IVPB] conventional dosing). Spin down and send 1 mL of serum refrigerated.
Note:
1. Date and time of draw are required on request form and specimen for processing.
2. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.
3. Indicate serum and trough/pre-dose on request form.
4. Label specimen appropriately (serum and as trough/pre-dose).
Alternate:
Plasma
Draw blood in a mint green-top (heparin) gel tube(s) immediately prior to next dose (intravenous piggyback [IVPB] conventional dosing). Spin down and send 1 mL of heparinized plasma refrigerated.
Note: 1. Date and time of draw are required on request form and specimen for processing.
2. Indicate plasma and trough/pre-dose on request form.
3. Label specimen appropriately (plasma and as trough/pre-dose).
Sample Stability Information:
Specimens removed from the cells, clot, or gel may be stored up to one week at 2 to 8°C.
If testing will be delayed more than one week, specimens should be stored frozen (< -10°C).
Specimen Transport Temperature
Refrigerate/Frozen OK/Ambient NO
Reference Values
Therapeutic range: 0.0-2.0 mcg/mL
Critical value >2.0 mcg/mL
Alerting category: Always called within 1 hour
Note: Sagamicin, sisomycin, and netilmicin interfere with this assay. Concurrent treatment with other nephrotoxic drugs may increase nephrotoxicity.
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
80170