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Test Code LAB2900 Hemoglobin Identification, Blood

Reporting Title

Hgb Identification

Methodology

Bio-Rad, High Pressure Liquid Chromatography (HPLC) Used for Identification and Quantitation of Hemoglobin

Alkaline and acid gel electrophoresis performed if warranted.

Hemoglobin-S Solubility testing performed to confirm the presence of Hgb-S.

Performing Laboratory

OhioHealth Laboratory Services-RMH Core Hematology

Specimen Requirements

Draw blood in a lavender-top (EDTA) tube(s), and send EDTA whole blood. Mix by gently inverting 8 times. Forward unprocessed whole blood refrigerated or ambient.

Note:  Please provide recent transfusion history, patient’s age, race, and diagnosis.

Specimen Transport Temperature

Refrigerate OK/Ambient OK/ Frozen NO

Reference Values

Hemoglobin A:  96-100%

Hemoglobin A2: 1.5-3.7%

Hemoglobin F 0.8-2.0%

See individual test listings for other reference values.

Day(s) Test Set Up

Tuesday, Wednesday, Friday

Test Classification and CPT Coding

83021 - hemoglobin Identification

83020 - confirmation by electrophoresis (if appropriate)

85660 - sickle cell screen, solubility test (if appropriate)