Test Code LAB30 Phenobarbital, Plasma or Serum
Additional Codes
46290
Reporting Title
Phenobarb
Methodology
Roche Diagnostics- RMH, GMH, MH
Used to monitor dose and overdose of phenobarbital, an anticonvulsant.
VWH Lab Only:
PHE reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Drug Calibrator 1, is intended for quantitative determination of Phenobarbital concentration in human serum or plasma.
Performing Laboratory
OhioHealth Laboratory Services - RMH, GMH, MH and VWH Core Laboratories
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. Specimen is usually drawn just prior to next oral dose.
Note:
1. Draw times should be consistent.
2. Date and time of draw is required on request form and specimen for processing.
3. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.
4. Indicate serum on request form.
5. Label specimen appropriately (serum).
Alternate (Preferred for VWH Lab):
Plasma
Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. Specimen is usually drawn just prior to next oral dose.
Note:
1. Draw times should be consistent.
2. Date and time of draw is required on request form and specimen for processing.
3. Indicate plasma on request form.
4. Label specimen appropriately (plasma).
VWH Lab:
5.Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells within two hours from the time of collection.
6. Separated serum or plasma should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, serum or plasma should be stored at +2°C to +8°C for up to 48 hrs.
Roche Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum or plasma | Refrigerated | 7 days capped |
Ambient | 7 days capped | |
Frozen | 12 months capped |
Specimen Transport Temperature
Refrigerate/Ambient OK/Frozen OK
Reference Values
Therapeutic range: 15-40 mcg/mL
Critical value >40 mcg/mL
Alerting category: Always called within 1 hour
Note: Both primidone and mephobarbital are metabolized to phenobarbital.
VWH Lab Only:
15–40 µg/mL
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
80184