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Test Code LAB306 Factor VIII Assay, Blood or Plasma

Important Note

Please see the Coagulation Limitations under resources for effect of Anticoagulant drugs in this test.

Requests after 2pm on Saturday and Sunday require a Pathologist's approval.

Additional Codes

45585

Reporting Title

Factor VIII (8) Assay

Methodology

End point Clot Detection
Used to detect factor VIII deficiency.

Performing Laboratory

OhioHealth Laboratory Services-RMH Core Coagulation

Specimen Requirements

Submit only 1 of the following specimens:

 

Riverside Inpatients or Riverside Campus draw sites:

Testing must be performed within 4 hours of draw if submitting unprocessed whole blood.

Draw a full, light blue-top (citrate) tube; and send citrated whole blood. Invert several times to mix blood. Forward unprocessed whole blood promptly at ambient temperature only. Do not refrigerate. 

Note:

1. This assay requires its own specimen.

2. Gross hemolysis, icterus, and lipemia may affect results.

3. Indicate whole blood on request form.

4. Label specimen appropriately (whole blood).

 

Outpatients/Outreach Clients:

Plasma-Frozen

Draw a full, light blue-top (citrate) tube. Spin down, remove plasma, spin plasma again, and place 1 mL of citrated platelet-poor plasma in plastic vial. (Glass vial is not acceptable.) Pour-off vial should be no more than 2/3 full. Send specimen frozen.

Note:

1. This assay requires its own specimen.

2. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious

    results.

3. Gross hemolysis, icterus, and lipemia may affect results.

4. Indicate plasma on request form.

5. Label specimen appropriately (plasma).

NOTE: If multiple factor assays are requested, 2-3 (1ml) aliquots are required.

 

Specimen Transport Temperature

Ambient OK/Refrigerate NO/Frozen NO-Blood
Frozen OK/Refrigerate NO/Ambient NO-Plasma

Reference Values

51-154%

Critical value: <5%

Alerting category: First instance within 1 hour (initial result only, routine on results thereafter.

Day(s) Test Set Up

Monday through Friday (after 2 p.m. and on Saturdays and Sundays performed on emergency basis only)

Requests after 2pm on Saturday and Sunday require a Pathologist's approval.

Test Classification and CPT Coding

85240