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Test Code LAB31 Phenytoin, Total, Plasma or Serum

Reporting Title

Phenytoin

Methodology

Roche Diagnostics: RMH, BH, DH, GMC, GMH, MGH, MH, OBH, SH, VWH Core Laboratories

Used to monitor dose and overdose of phenytoin, an anticonvulsant.

 

 

Performing Laboratory

OhioHealth Laboratory Services - RMH, BH, DH, GMC, GMH, MGH, MH, OBH, SH and VWH Core Laboratories

Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

 

Submit only 1 of the following specimens:

 

Preferred:

Serum

Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. Specimen is usually drawn 2 hours post infusion or 15 minutes before dose for intravenous piggyback (IVPB) or intravenous push (IVP). For an oral dose, specimen is drawn immediately prior to next dose.

Note:

1. Patients receiving fosphenytoin should wait at least 2 hours after an intravenous administration and 4 hours after an intramuscular administration before having blood drawn.

2. Date and time of draw is required on request form and specimen for processing.

3. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

4. Indicate serum and if draw is pre- or post-dose on request form.
5. Label specimen appropriately (serum and as pre- or post-dose).

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Serum or plasma Refrigerated 4 days capped
  Ambient  4 days capped
  Frozen  2 months capped

 

Specimen Transport Temperature

Refrigerate/Frozen OK/Ambient OK

Reference Values

Therapeutic concentration

Neonates:  6-15 mcg/mL

Children and adults:  10-20 mcg/mL

Critical values Neonates:  >15 mcg/mL  Children and adults:  >25 mcg/mL

Alerting category: Always called within 1 hour

Note:  Phenobarbital may increase or decrease phenytoin levels. A free phenytoin level can identify protein-binding problems in uremic patients.

 

 

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

80185