Test Code LAB31 Phenytoin, Total, Plasma or Serum
Reporting Title
Phenytoin
Methodology
Roche Diagnostics: RMH, BH, DH, GMC, GMH, MGH, MH, OBH, SH, VWH Core Laboratories
Used to monitor dose and overdose of phenytoin, an anticonvulsant.
Performing Laboratory
OhioHealth Laboratory Services - RMH, BH, DH, GMC, GMH, MGH, MH, OBH, SH and VWH Core Laboratories
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. Specimen is usually drawn 2 hours post infusion or 15 minutes before dose for intravenous piggyback (IVPB) or intravenous push (IVP). For an oral dose, specimen is drawn immediately prior to next dose.
Note:
1. Patients receiving fosphenytoin should wait at least 2 hours after an intravenous administration and 4 hours after an intramuscular administration before having blood drawn.
2. Date and time of draw is required on request form and specimen for processing.
3. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.
4. Indicate serum and if draw is pre- or post-dose on request form.
5. Label specimen appropriately (serum and as pre- or post-dose).
Roche Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum or plasma | Refrigerated | 4 days capped |
| Ambient | 4 days capped | |
| Frozen | 2 months capped |
Specimen Transport Temperature
Refrigerate/Frozen OK/Ambient OK
Reference Values
Therapeutic concentration
Neonates: 6-15 mcg/mL
Children and adults: 10-20 mcg/mL
Critical values Neonates: >15 mcg/mL Children and adults: >25 mcg/mL
Alerting category: Always called within 1 hour
Note: Phenobarbital may increase or decrease phenytoin levels. A free phenytoin level can identify protein-binding problems in uremic patients.
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
80185