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Test Code LAB321 Prothrombin Time (PT) Mixing Studies, Blood or Plasma

Additional Codes

46392

Reporting Title

PT Mixing Study

Methodology

Equal amounts of patient and normal plasma are combined. A PT is performed before and if needed, after a 2-hour, 37° C incubation.

Used to detect the presence of an inhibitor factor deficiency.

Performing Laboratory

OhioHealth Laboratory Services-RMH Core Coagulation

Specimen Requirements

Submit only 1 of the following specimens:

 

Riverside Inpatient and Riverside Campus draw sites:

Testing must be performed within 4 hours of draw if submitting unprocessed whole blood.

Draw 4 full, light blue-top (citrate) tubes; and send citrated whole blood. Invert several times to mix blood. Forward unprocessed whole blood promptly at ambient temperature only. Do not refrigerate.

Note:  1. This test is only performed when patient’s PT is  above upper limit of PT normal range.

2. Indicate whole blood on request form.

3. Label specimens appropriately (whole blood).

 

Outpatient/Outreach Clients

Plasma-Frozen

Draw 3 full, light blue-top (citrate) tubes. Spin down, remove plasma, spin plasma again, and place citrated platelet-poor plasma in 4 plastic vials each containing 1 mL. (Glass vial is not acceptable.) Pour-off vials should be no more than 1/3 full. Send specimen frozen.

Note:  1. This test is only performed when patient’s PT is above upper limit of PT normal range.

2. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

3. Indicate plasma on request form.

4. Label specimens appropriately (plasma).

NOTE: If ordered in conjunction with APTT Mixing Study, only 4 aliquots required for both tests.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO-Blood

Frozen/Refrigerate NO/Ambient NO-Plasma

Reference Values

Negative

The specific type of inhibitor present cannot be determined by this screen. Further testing is required to identify type of inhibitor present. Recommend quantitative inhibitor studies.

Day(s) Test Set Up

Monday through Friday

Test Classification and CPT Coding

85611

Additional Information

 If a Thrombin Time is needed, then one will be ordered, performed and resulted.