Test Code LAB326 Activated Partial Thromboplastin Time (APTT) Mixing Study, Blood or Plasma
Additional Codes
45116
Reporting Title
APTT Mixing Study
Methodology
Equal amounts of patient and normal plasma are combined. An APTT is performed before and if needed, after a 120-minute 37° C incubation.
Used to detect the presence of an inhibitor/factor deficiency.
Performing Laboratory
OhioHealth Laboratory Services-RMH Core Coagulation
Specimen Requirements
Submit only 1 of the following specimens:
Riverside Inpatients and Riverside Campus draw sites:
Testing must be performed within 4 hours of draw if submitting unprocessed whole blood.
Draw 3 full, light blue-top (citrate) tubes; and send citrated whole blood. Invert several times to mix blood. Forward unprocessed whole blood promptly at ambient temperature only. Do not refrigerate.
Note: 1. Gross hemolysis, lipemia, and icteric specimen may affect results.
2. The presence of heparin will cause false-positive results. Patient must be heparin free for a minimum of 12 hours prior to draw.
3. This test is only performed when patient’s APTT is above the upper limit of the normal APTT range.
4. Indicate whole blood on request form.
5. Label specimens appropriately (whole blood).
Outpatients/Outreach clients:
Plasma-Frozen
Draw 4 full, light blue-top (citrate) tubes. Spin down, remove plasma, spin plasma again, and place citrated platelet-poor plasma into 4 plastic vials each containing 1 mL. (Glass vial is not acceptable.) Pour-off vials should be no more than 2/3 full. Send specimen frozen.
Note: 1. Gross hemolysis, lipemia, and icteric specimen may affect results.
2. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
3. The presence of heparin will cause false-positive results. Patient must be heparin free for a minimum of 12 hours prior to draw.
4. This test is only performed when patient’s APTT is above the upper limit of the normal APTT range.
5. Indicate plasma on request form.
6. Label specimens appropriately (plasma).
NOTE: If ordered in conjuction with the PT Mixing Study, 4 aliquot tubes are required for both tests.
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO-Blood
Frozen/Refrigerate NO/Ambient NO-Plasma
Reference Values
Negative
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
85732
Additional Information
If a Thrombin Time is needed, then one will be ordered, performed and resulted.