Test Code LAB38 Tobramycin, Trough/Pre-Dose, Plasma or Serum
Additional Codes
46579
Reporting Title
Tobramycin, Pre-Dose
Methodology
Roche Enzymatic
Tobramycin, an antibiotic, has a narrow therapeutic index. It is particularly hazardous in patients with impaired renal function. The dose-serum level profile curve is unpredictable for peak serum levels and elimination half-life.
Performing Laboratory
OhioHealth Laboratory Services-RMH Core Laboratory
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s) immediately prior to
next dose (trough level). Spin down and send 1 mL of serum
refrigerated.
Note: 1. Date and time of draw are
required on request form and specimen for processing.
2. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.
3. Indicate serum and trough/pre-dose on request form.
4. Label specimen appropriately (serum and as trough/pre-dose).
Alternate:
Plasma
Draw blood in a mint green-top (heparin) gel tube(s) immediately prior to next dose (trough level). Spin down and send 1 mL of heparinized plasma refrigerated.
Note: 1. Date and time of draw are required on request form and specimen for processing.
2. Indicate plasma and trough/pre-dose on request form.
3. Label specimen appropriately (plasma and as trough/pre-dose).
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum or plasma | Refrigerated | 3 days capped |
Ambient | 3 days capped | |
Frozen | 1 month capped |
Specimen Transport Temperature
Refrigerate/Frozen OK/Ambient NO
Reference Values
Therapeutic range: 0-2.0 mcg/mL
Critical value >2.0 mcg/mL
Alerting category: Always called within 1 hour
Note: 1. Kanamycin, dibekacin, and amikacin cause falsely elevated results.
2. Trough levels more closely correspond to tissue levels and are less prone to sampling errors.
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
80200