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Test Code LAB472 Hepatitis Bs Antibody (Anti-HBs), Qualitative

Reporting Title

Hepatitis Bs Ab

Methodology

Roche Diagnostics Electrochemiluminescence Immunoassay

Useful for qualitative determination of total antibodies to hepatitis B surface antigen. Used to determine immune response following vaccination and to detect sero conversion with recovery in cases of hepatitis B infection.

Performing Laboratory

OhioHealth Laboratory Services-RMH Special Testing

Specimen Requirements

Preferred;

Draw blood in a gold-top serum gel tube(s). (Hemolyzed specimen is not acceptable.) Spin down and send 1 mL of serum refrigerated.  A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

Alternate:

Plasma from K2 EDTA is acceptable

 Heterophile antibodies may interfere with this assay.

 

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 6 days
  Frozen  3 months
     
Plasma Refrigerated 2 days
  Frozen 3 months

 

Specimen Transport Temperature

Refrigerate: OK

Frozen: OK

Ambient: NO

Reference Values

Unvaccinated, unexposed to hepatitis B:  negative

Note:  1. This assay does not distinguish between vaccine-induced antibodies and infection-induced antibodies. A total antibody hepatitis B core assay may help resolve this.

2. A negative result does not indicate that patient was not vaccinated or was not infected with hepatitis B virus.

Day(s) Test Set Up

Monday through Friday: 24/7

Saturday and Sunday: Day shift

Test Classification and CPT Coding

86706