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Test Code LAB481 Methotrexate

Additional Codes

46132

Reporting Title

Methotrexate

Methodology

ARK diagnostics Homogeneous Enzyme Immunoassay

Used to monitor methotrexate therapy.

Performing Laboratory

OHLS- RMH Core Lab

Specimen Requirements

Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. 

Also Acceptable:

Draw blood in a Gold top (no anticoagulant).  Spin down and send 2 mL of serum refrigerated. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

NOTE: Note exact time of collection

 

Specimen Stability Information

Specimen Type Temperature Time
Plasma/Serum Refrigerated 14 days
  Ambient   
  Frozen  Stable

 

Specimen Transport Temperature

Refrigerate/Frozen OK/Ambient NO

Reference Values

Following a 4-6 hour IV infusion:

at 24 hours-Potentially toxic if >5.0 micromol/L

at 48 hours-Potentially toxic if >0.5 micromol/L

at 72 hours-Potentially toxic if >0.05 micromol/L

Due to different protocols using this drug, contact the primary attending physician.

Critical value call all regardless of result

Alerting category: Always call within 1 hour

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

80299