Test Code LAB50 Bilirubin, Total, Plasma or Serum
Reporting Title
Bilirubin, Total
Methodology
Note: See LAB52 “Bilirubin, Direct, Plasma or Serum” to order only direct bilirubin.
Testing Algorithm:
A Total Bilirubin >1.3 mg/dL will reflex LAB52 “Direct Bilirubin", Plasma or Serum” at an additional charge.
Method Name:
Roche Diagnostics, Jendrassik-Grof -RMH, GMC, DH, DMH, GMH, WECC, PMC, GCMH, OBH, MGH, MH, HMH, SH
General Information:
Bilirubin is formed by the reticuloendothelial system during the normal and abnormal destruction of red cells. Testing for bilirubin is useful in the diagnosis of liver disease and hemolytic anemia.
VWH Lab Only:
TBIL reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Bilirubin Calibrator, is intended for quantitative determination of Total Bilirubin concentration in human serum or plasma.
Performing Laboratory
OhioHealth Laboratory Services - RMH, GMC, DH, DMH, MGH, GMH, HMH, WECC, PMH, OBH, MH, SH, GCMH, BH and VWH Core Laboratories
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated.
Note:
1. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.
2. Indicate serum on request form.
3. Label specimen appropriately (serum).
Alternate (Preferred for VWH Lab):
Plasma
Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. Protect specimen from light.
Note:
1. Indicate plasma on request form.
2. Label specimen appropriately (plasma).
VWH Lab:
3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells within two hours from the time of collection.
4. Separated serum or plasma should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, serum or plasma should be stored at +2°C to +8°C for up to 48 hrs.
Roche Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum or plasma | Refrigerated | 7 days |
Ambient | 2 days | |
Frozen | 6 months |
PROTECT FROM LIGHT
Specimen Transport Temperature
Refrigerate/Ambient NO/Frozen NO
Reference Values
0-1 days: 2.0-6.0 mg.dL
2-3 days: 3.0-9.0 mg/dL
4-30 days: 4.0-12.0 mg/dL
≥30 days: 0.0-1.3 mg/dL
Critical value up to 30 days: >18.0 mg/dL
Alerting category: Always called alert within 1 hour (initial result only)
VWH Lab Only:
0.3 – 1.2 mg/dL
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
82247 - bilirubin, total
82248 - bilirubin, direct (if appropriate)