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Test Code LAB50 Bilirubin, Total, Plasma or Serum

Important Note

Note:  See LAB52 “Bilirubin, Direct, Plasma or Serum” to order only direct bilirubin.

Reporting Title

Bilirubin, Total

Methodology

Note:  See LAB52 “Bilirubin, Direct, Plasma or Serum” to order only direct bilirubin.

Testing Algorithm:

A Total Bilirubin >1.3 mg/dL will reflex  LAB52 “Direct Bilirubin",  Plasma or Serum” at an additional charge.

Method Name:

Roche Diagnostics, Jendrassik-Grof -RMH, GMC, DH, DMH, GMH, WECC, PMC, GCMH, OBH, MGH, MH, HMH, SH

General Information:

Bilirubin is formed by the reticuloendothelial system during the normal and abnormal destruction of red cells. Testing for bilirubin is useful in the diagnosis of liver disease and hemolytic anemia.

 

VWH Lab Only: 

TBIL reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Bilirubin Calibrator, is intended for quantitative determination of Total Bilirubin concentration in human serum or plasma.

Performing Laboratory

OhioHealth Laboratory Services - RMH, GMC, DH, DMH, MGH, GMH, HMH, WECC, PMH, OBH, MH, SH, GCMH, BH and VWH Core Laboratories

Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

 

Submit only 1 of the following specimens:

 

Preferred:

Serum

Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. 

Note:

1. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

2. Indicate serum on request form.

3. Label specimen appropriately (serum).

 

Alternate (Preferred for VWH Lab): 

Plasma

Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. Protect specimen from light.

Note: 

1. Indicate plasma on request form.

2. Label specimen appropriately (plasma).

VWH Lab:

3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells within two hours from the time of collection.

4. Separated serum or plasma should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, serum or plasma should be stored at +2°C to +8°C for up to 48 hrs. 

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Serum or plasma Refrigerated 7 days
  Ambient  2 days
  Frozen  6 months

PROTECT FROM LIGHT

 

Specimen Transport Temperature

Refrigerate/Ambient NO/Frozen NO

Reference Values

0-1 days:  2.0-6.0 mg.dL

2-3 days:  3.0-9.0 mg/dL

4-30 days:  4.0-12.0 mg/dL

≥30 days:  0.0-1.3 mg/dL

Critical value up to 30 days:  >18.0 mg/dL

Alerting category: Always called alert within 1 hour (initial result only)

 

VWH Lab Only: 

0.3 – 1.2 mg/dL

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

82247 - bilirubin, total

82248 - bilirubin, direct (if appropriate)