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Test Code LAB52 Bilirubin, Direct, Plasma or Serum

Reporting Title

Bilirubin, Direct

Methodology

Roche Diagnostics, Jendrassik-Grof   RMH, GMC,DH, DMH, GMH, WECC, PMC, GCMH, BH, OBH, MGH, MH, HMH, SH

Note:  See LAB50 “Bilirubin, Total, Plasma or Serum” for total bilirubin with reflex to direct bilirubin.

Bilirubin is formed by the reticuloendothelial system during the normal and abnormal destruction of red cells. Testing for bilirubin is useful in the diagnosis of liver disease and hemolytic anemia.

 

VWH Lab Only: 

DBIL reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Bilirubin Calibrator, is intended for quantitative determination of direct (conjugated) bilirubin concentration in human serum or plasma.

Performing Laboratory

OhioHealth Laboratory Services-RMH, GMC, DH, DMH, MGH, GMH, HMH, WECC, PMH, OBH MH, SH, GCMH, BH and VWH Core Laboratories

Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

 

Submit only 1 of the following specimens:

 

Preferred:

Serum

Draw blood in a gold-top or a speckled-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated. 

Note:  1. Plain, red-top tube(s) is also acceptable.

2. Indicate serum on request form.

3. Label specimen appropriately (serum).

 

Alternate (Preferred for VWH Lab): 

Plasma

Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated. Protect specimen from light.

Note:  1. Indicate plasma on request form.

2. Label specimen appropriately (plasma).

VWH Lab:

3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells within two hours from the time of collection.

4. Separated serum or plasma should not remain at room temperature longer than 8 hours. If assays are not completed within 8 hours, serum or plasma should be stored at +2°C to +8°C. Refrigerated specimens are stable for 48 hrs. 

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Serum or plasma Refrigerated 7 days
  Ambient  2 days
  Frozen  6 months

PROTECT FROM LIGHT

Specimen Transport Temperature

Refrigerate/Ambient NO/Frozen NO

VWH Lab: RefrigerateOK/Ambient OK/Frozen NO

Reference Values

0-22 days:  0.0-1.5 mg/dL

≥23 days:  0.0-0.40 mg/dL

 

VWH Lab Only:

 Serum or Plasma: 0.0 – 0.2 mg/dL

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

82248