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Test Code LAB535 Vitamin D, Total, 25-Hydroxy, Serum

Important Note

Order this test if an order for Vitamin D is received- do not order 1-25 DiHydroxyvitamin D unless it is specifically listed on the order.   This is the Routine Screening test for Vitamin D deficiency.

Reporting Title

Vitamin D, Total, 25 OH

Methodology

DiaSorin LIAISON®, Competitive Two-Step Chemiluminescence Assay at Riverside

 

Roche Diagnostics, Elecsys®/Electrochemiluminescence Immunoassay at OBH and MH Core Laboratories

 

25 OH Vitamin D is the preferred initial screening test for assessing Vitamin D status. It is indicated for the diagnosis of vitamin D deficiency, for monitoring Vitamin D replacement therapy and for diagnosing Vitamin D hypervitaminosis.

Note:  Ordering the correct Vitamin D test is often confusing since several tests have variations of “Vitamin D” in the title. Vitamin D, Total, 25 OH is the preferred screening test for Vitamin D status. 1-25 dihydroxyvitamin D is specifically used to evaluate the Vitamin D status in patients with kidney disease and other metabolic disorders. It is not a screening or monitoring test.

Performing Laboratory

OhioHealth Laboratory Services-RMH Special Testing and MH Core Laboratories

Specimen Requirements

SERUM ONLY (RMH)

Draw blood in a gold-top serum gel tube (s).  Send refreigerated or spin down and send 0.5 mL of serum refrigerated.

 

Note:

A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable.

MH and OBH Laboratories also accept plasma from a mint tube. 

 

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 5 days
     
  Frozen  Stable

 

Specimen Transport Temperature

Refrigerate/Frozen/Ambient NO

Reference Values

TOTAL 25-HYDROXYVITAMIN D2 AND D3 (25-OH-VitD)

<10 ng/mL (deficiency)

10-30 ng/mL (insufficiency)

30-100 ng/mL (sufficiency)

>100 ng/mL (toxicity)

 

OBH Roche Cobas Pro

TOTAL 25-HYDROXYVITAMIN D2 AND D3 (25-OH-VitD)

<10 ng/mL (deficiency)

10-20 ng/mL (insufficiency)

20-100 ng/mL (sufficiency)

>100 ng/mL (toxicity)

Day(s) Test Set Up

OBH and MH  Monday through Saturday; Continuously

RMH Special Testing: Monday through Friday Continuous

Saturday and Sunday Day shift only

Test Classification and CPT Coding

82306