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Test Code LAB834 Prothrombin G20210A Mutation, Blood

Reporting Title

Prothrombin G20210A Mutation

Methodology

Direct Mutation Analysis

Direct variant analysis using PCR amplification, signal generation, and release by cleavage of sequence specific alleles.(Invader Factor II, Invader Plus Chemistry. Hologic, Inc; 2011)

Performing Laboratory

Mayo Clinical Laboratories

Specimen Requirements

Container/Tube:

Preferred: Yellow top (ACD solution A or B)

Acceptable: Lavender top (EDTA) or light blue top (sodium citrate)

Specimen Volume: Full tube

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Additional Information: Can be combined with other molecular coagulation tests:

-F5DNA / Factor V Leiden (R506Q) Mutation, Blood

 

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood Refrigerated 14 days
  Ambient  7 days
  Frozen  14 days

 

Specimen Transport Temperature

Ambient OK/Refrigerate OK/Frozen OK

Reference Values

Negative, no mutation detected

Day(s) Test Set Up

Monday through Friday; 12 p.m

Turn-around-time: 3 days

Test Classification and CPT Coding

81240-F2 (prothrombin, coagulation factor II) (eg, hereditary hypercoagulability) gene analysis, 20210G->A variant