Home
HelpTest Code LAB876 Tacrolimus, Blood
Reporting Title
Tacrolimus, Blood
Useful For
Monitoring whole blood tacrolimus concentration during therapy, particularly in individuals coadministered CYP3A4 substrates, inhibitors, or inducers
Adjusting dose to optimize immunosuppression while minimizing toxicity
Evaluating patient compliance
Performing Laboratory
OhioHealth Laboratory Services- RMH Special Testing
Specimen Requirements
Whole Blood EDTA
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Draw blood immediately before a schedule dose.
2. Do not centrifuge.
3. Send specimen in original tube.
Additional Information: Therapeutic range applies to trough specimens drawn immediately prior to a.m. dose.
Specimen Minimum Volume
1 mL
Specimen Stability
Specimen Stability Information
|
Specimen Type |
Temperature |
Time |
Special Container |
|
Whole Blood EDTA |
Refrigerated (preferred) |
7 days |
|
|
|
Ambient |
5 days |
|
|
|
Frozen |
3 months |
Reference Values
5.0-15.0 ng/mL (Trough)
Target trough concentrations may vary depending on the type of transplant, concomitant immunosuppression therapy, or clinical protocol.
Specimens with biotin concentrations up to 100 ng/mL demonstrated ≤ 10 % bias in results. Biotin concentrations greater than 100 ng/mL led to higher positive bias Tacrolimus results. Some studies have shown that serum concentrations of biotin can reach up to 355 ng/mL within the first hour after biotin ingestion for subjects consuming supplements of 20 mg biotin per day18 and up to 1160 ng/mL for subjects after a single dose of 300 mg biotin.
Day(s) Performed
Monday through Friday Continuous
Saturday and Sunday Day shift only
Test Classification and CPT Coding
CPT 80197
Method Name
Roche Diagnostics Electrochemiluminescence Immunoassay