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Test Code LAB90000 14-3-3 Antigen, CSF

Important Note

Complete neurological clinical history and findings must be provided. 

Infectious

Reporting Title

14-3-3 Antigen, CSF

Specimen Collection

Collect CSF by lumbar puncture. Discard the first 2 ml of CSF that flows from the tap. Collect 2-5 ml of clear, colorless CSF for testing. Bloody CSF cannot be accurately tested.

A minimum of 2.0ml is required for testing

Specimen Requirements

2 mL CSF 

 

Patient must be 12 years of age or older.

Specimen Transport and Storage

Freeze CSF immediately after collection or as soon as possible. Store CSF at -80°C (or at least -20°C) until shipping the specimen on dry ice.

 

Specimen stability: 

Frozen: Indefinitely.

Specimen Transport Temperature

Frozen (preferred)

Performing Laboratory

NPDPSC- National Prion Disease Pathology Surveillance Center

2085 Adelbert Rd, Rm 419
Cleveland, Ohio 44106

PH: 216-368-0587

Day(s) Performed

Mon-Fri, excluding federal holidays

Methodology

Quantitative Enzyme-Linked Immunosorbent Assay (ELISA). 
Real-Time Quaking-Induced Conversion (RT-QuIC)ii.

Reference Values

RT-QuIC: Negative
 

Total TAU (ELISA): 0-1149 pg/ml
 

14-3-3 GAMMA (ELISA): <30-1999 AU.

CPT Coding

83520

Additional Information

May aid in diagnosis of prion disease.
Assumes that the patient has a history of a rapid neurodegenerative condition and does not have several conditionsi that might compromise the test results.

 

Prion marker testing prion panel (14-3-3, Tau, and RT-QuIC) testing. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded. NPDPSC measures Total Tau in CSF, which is markedly elevated with prion disease. RT-QuIC (real-time quaking-induced conversion) is a high specificity test that used to detect the abnormal prion protein.