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Test Code LAB90283 Immunodeficiency Panel T and B, Blood

Important Note

T- and B-Cell Quantitation by Flow Cytometry, Blood

Reporting Title

Immunodeficiency Panel

Methodology

Flow Cytometry

Note:  This assay is not for diagnosing lymphocytic malignancies. Useful for monitoring CD4 counts and assessing immune deficiencies.

Performing Laboratory

OhioHealth Laboratory Services-RMH Special Testing

Specimen Requirements

Draw blood in a lavender-top (EDTA) tube(s). (Grossly hemolyzed specimen is not acceptable.) Mix gently by inverting 8 times.

Do not centrifuge.

Forward unprocessed whole blood promptly at ambient temperature only. 

Sample is stable at room temperature for 48 hours. Do not refrigerate or freeze.

Specimen Transport Temperature

Ambient OK/Refrigerate NO/Frozen NO

Reference Values

IMMUNODEFICIENCY

% CD19 (B-cells):  6-22%

Absolute CD19:  89-541 cells/µL

% CD3 (T-cells):  62-85%

Absolute CD3:  740-2,380 cells/µL

% CD4 (helper cells):  32-58%

Absolute CD4:  430-1,580 cells/µL

% CD8 (suppressor cells):  15-41%

Absolute CD8:  232-1,030 cells/µL

% CD16 + % CD56:  4-25%

Absolute CD16 + CD56:  70-480 cells/uL 

CD4/CD8 ratio:  0.97-3.64

Day(s) Test Set Up

Monday through Friday

Saturday; if received before 10 a.m.

Test Classification and CPT Coding

86355 - B cells; total count

86357 - natural killer cells; total count

86359 - T cells; total count

86360 - T cells; absolute CD4 and CD8 count, including ratio