Test Code LAB90366 Culture, Eye (Surgical), Mycobacterium
Additional Codes
44228
Reporting Title
Eye (Superficial) AFB Culture
Methodology
Conventional Culture Method/Nucleic Acid Probe when Appropriate
Performing Laboratory
OhioHealth Laboratory Services-RMH Microbiology
Specimen Requirements
Submit only 1 of the following specimens:
Non-Swab Specimen
1. Collect specimen from affected area and place in a screw-capped, sterile container.
2. Label container with patient’s name (first and last), MR# or DOB, date and actual time of collection and type of specimen.
3. Maintain sterility and forward promptly at ambient temperature only.
Note: Specimen source is required on request form for processing.
Swab Specimen
Collect specimen from the affected area using a BD
ESwabTM as follows:
1. Take swab for culture before application of topical anesthetics
because topical anesthetics possess antimicrobial activity.
2. Cleanse skin around eye with mild antiseptic.
3. Gently remove makeup and ointment with sterile cotton and saline.
4.Collect the specimen using the swab provided in the BD ESwab Collection and Transport System. Pass swab
2 times over lower conjunctiva. Avoid eyelid border and lashes (culture these separately in a similar fashion, if indicated). Be sure not to touch below the red breakpoint line to avoid contamination.
5. Aseptically unscrew and remove the cap from the tube.
6. Insert the swab into the tube and bend the swab shaft at the breakpoint indicated by the red line marked on the swab shaft against the tube to break the shaft. Discard the broken handle part of the swab shaft.
7. Replace the cap on the tube and secure it tightly.
8. Label tube with patient’s name (first and last), MR# or DOB, date and actual time of collection, and type of specimen.
9. Maintain sterility and forward promptly at ambient
temperature only.
Note: Specimen source is required on request
form for processing.
ESwab is acceptable up to 48 hours after collection
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Reference Values
No growth after 8 weeks
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
87116 - culture, Mycobacterium
87206 - smear
87149 - identification by nucleic acid probe (if appropriate)