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Test Code LAB90499 Troponin T

Reporting Title

Troponin T

Methodology

Roche Diagnostics, 5th Generation high sensitive cardiac troponin T assay.  (hs-cTnT)Electrochemiluminescence Immunoassay

In patients with elevated troponin levels or chest pain of less than 3 hours duration, serial testing is necessary to rule out acute events. The recommended interval for serial testing is 0 (baseline) and 2 hours. A delta troponin value will be included on serial troponin results between 2 and 12 hours apart. In collaboration with the OhioHealth Heart and Vascular Services Clinical Guidance Council, the laboratory has developed algorithms using data from our own patient population. Interpretive comments based on these algorithms will be part of the results.

 

VWH Lab Only:

The Access AccuTnI assay is a two-site immunoenzymatic (“sandwich”) assay. Monoclonal anti-cTnI antibody conjugated to alkaline phosphatase is added to a reaction vessel along with a surfactant-containing buffer and sample. After a short incubation, paramagnetic particles coated with monoclonal anti-cTnI antibody are added. The human TnI binds to the anti-cTnI antibody on the solid phase, while the anti-cTnI antibody – alkaline phosphatase conjugate materials bound to the solid phase are held in a magnetic field while unbound materials are washed away.Then, the chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of CTnI in the sample. The amount of analyte in the sample is determined from a strored multi-point calibration curve.

Performing Laboratory

OhioHealth Laboratory Services - RMH, GMC, DH, DMH, GMH, WECC, PMH, GCMH, BH, OBH, MGH, MH, HMH, SH and VWH Core Laboratories

Specimen Requirements

 

Plasma Only MINT GREEN TUBE

Mint Green (Li-heparin) plasma only. or Li Heparin green top tube no gel.  (Hemolyzed specimen is not acceptable.) Spin down and send 1 mL of heparinized plasma refrigerated.

Note:  1. Patients receiving therapy with mouse monoclonal antibodies may have erroneous results.

Due to interference, patients receiving high doses of biotin (>5 mg/day) should wait at least 8 hours after dose before having blood drawn.

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Plasma Refrigerated 24 hours
  Ambient   
  Frozen  12 months

 

 

VWH Lab Only:

1. Plasma should be physically separated from contact with cells as soon as possible with a maximum time limit of two hours from the time of collection.

2. Specimen stability: Ambient 8 hrs, Refrigerated 48 hrs

 

 

Specimen Transport Temperature

Refrigerate: OK

Ambient: OK

Frozen NO

Reference Values

Female <6 - 14 ng/L

Male <6 - 22 ng/L

  • A comment will be added to Troponin T orders on patients registered as Unknown for gender at the time of testing. The result will flag as critical >14 ng/L. A comment will display for the reference range: Patient sex not reported at the time of testing. Ref Range: Female ≤ 14; Male ≤22. 

 

Delta Troponin T (Abnormal indicates a clinically significant change in troponin concentration)

≤7 ng/L if initial troponin ≤ 35 ng/L

< 20% if initial troponin >35 ng/L

 

Critical Values

First time troponin value above the reference range

First time abnormal delta troponin

 

VWH Lab Only:

<0.06 ng/ml normal

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

84484