Test Code LAB93003 CSF Bacterial/Viral Detection by PCR
Reporting Title
CSF Bacterial/Viral Detection by PCR
Methodology
Aerobic culture and gram stain, with reflex to FilmArry amplified nucleic acid by PCR if indicated by parameters listed below:
- CSF Cell Count >5 WBC/mcL
- Pediatric patient <17 years old
- Special request by physician, if CSF ≤ 5 cells/mcL
This test is orderable for CSF obtained by lumbar puncture only. When ordering this test, a CSF Body Fluid Cell Count must also be ordered. The CSF Bacterial/Viral Detection by PCR BioFire FilmArray test is FDA approved for lumbar puncture CSF only, and targets the following organisms:
Bacteria |
Viruses |
Yeast |
Escherichia coli K1 |
Cytomegalovirus (CMV) |
Cryptococcus neoformans/gatti |
Haemophilus influenzae |
Enterovirus |
|
Listeria monocytogenes |
Human herpesvirus 6 |
|
Neisseria meningitidis |
Herpes simplex virus 1 |
|
Streptococcus agalactiae |
Herpes simplex virus 2 |
|
Streptococcus pneumoniae |
Human parechovirus |
|
|
Varicella-zoster virus |
|
Performing Laboratory
OhioHealth Laboratory Services-RMH Microbiology
Specimen Requirements
1. Aseptically collect 1 mL of spinal fluid in a screw-capped, sterile vial.
2. Label container with patients first and last name, MR# or DOB, date and actual time of collection and type of specimen.
3. Maintain sterility and forward promptly at ambient temperature.
Note: Specimen source is required on request form for processing.
Specimen Transport Temperature
Ambient/Refrigerate OK/Frozen NO
Transport information for sites other than Riverside Campus:
Receive test if previously ordered in LIS. Order test, print labels, and label specimen
Create a Room Temperature micro packing list only for this specimen and place it in the pocket of a special "green" biohazard bag with a label attached to the front specifying:
BIOFIRE STAT TEST, DELIVER DIRECTLY TO RMH MICRO LAB
Call a STAT courier if the next courier run is more than 60 minutes from the time of receipt
Reference Values
NOT DETECTED for all organisms included on the panel.
Culture follow-up: No growth after 72 hours
Day(s) Test Set Up
Monday through Sunday, Continuously
Test Classification and CPT coding
87070 - aerobic culture
87205 - Gram stain
87483 –CSF Filmarray PCR Panel (if appropriate)
87077 - definitive identification, each (if appropriate)
87147 - serotyping, each (if appropriate)
87181 - E-test, each (if appropriate)
87185 - enzyme detection, each (if appropriate)
87186 - susceptibility, minimum inhibitory concentration, each (if appropriate)