Sign in →

Test Code LAB93780 Strict Sperm Morphology, Slide

Additional Codes

MSTC1

Reporting Title

Strict Sperm Morphology, Slide

Methodology

Kruger Criteria Strict Morphology

Useful For Diagnosing male infertility Selecting the most cost-effective therapy for treating male-factor infertility Quantifying the number of germinal and WBCs per mL of semen

 

Performing Laboratory

Mayo Clinic Laboratories

Specimen Requirements

Specimen Type: Semen

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.

Container/Tube: Slides Specimen Volume: 2 slides-10 microL of liquefied semen on each slide

Collection Instructions:

1. If sperm concentration is <10 million/mL, centrifuge the specimen at 300 x G for 10 minutes before making slides.

2 Label 2 frosted slides in pencil with the patient's first and last name and the date of specimen collection. No adhesive labels.

3. Allow the semen to liquefy for 30 minutes.

4. Place 10 microL of liquefied semen on the label end of each slide, and evenly smear the specimen using a plain slide (this process is the same as making a blood smear).

5. Allow the smears to air dry for 15 minutes before placing both slides into 1 slide holder for shipment.

 

Specimen Transport Temperature

Ambient OK /Refrigerate NO/Frozen NO

Reference Values

Reference Values

Normal forms: ≥4.0%

Germinal cells/mL

<4 x 10(6) (normal)

≥4 x 10(6) (elevated germinal cells in semen are of unknown clinical significance)

WBC/mL

<1 x 10(6) (normal)

≥1 x 10(6) (elevated white blood cells in semen are of questionable clinical significance)

Interpretation

Categorizing sperm according to strict criteria based on measurements of head and tail sizes and shapes. Sperm with abnormalities in head, tail size, or shape may not be capable of completing critical steps in sperm transport and fertilization.

Day(s) Test Set Up

Monday through Friday; 3 p.m.

Send specimen Monday through Thursday only and not the day before a holiday

Test Classification and CPT Coding

89398