Test Code LAB94218 B-Type Natriuretic Peptide, VWH
Additional Codes
BNP
Reporting Title
B-Type Natriuretic Peptide - VWH
Methodology
The Access BNP test is a two-site immunoenzymatic (“sandwich”) assay. A sample is added to a reaction vessel with mouse monoclonal anti-human BNP antibody-alkaline phosphatase conjugate and paramagnetic particles coated with mouse omniclonal anti-human BNPantibody. BNP in human plasma binds to the immobilized anti-BNP on the solid phase, while the mouse anti-BNP conjugate reacts specifically with bound BNP. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. A chemiluminescent substrate, Lumi-Phos* 530, is added to the reaction vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of BNP in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.
Performing Laboratory
VWH Laboratory, Chemistry
Specimen Requirements
Plasma (EDTA) is the required sample. Other specimen types have not been evaluated.
Observe the following recommendations for handling, processing, and storing blood
samples 10:
- Collect all blood samples observing routine precautions for venipuncture.
- Mix the blood specimen by gently inverting the tube several times.
- Keep tubes stoppered at all times.
Samples should be tested as soon as possible after collection. However, if it is not possible
to test the samples immediately, the following is recommended:
- Whole blood samples may be stored at room temperature or refrigerated for up to 7 hours prior to testing.
- Plasma samples may be stored 24 hours refrigerated prior to testing.
Specimen Stability
Refrigerated 24 hrs
Ambient 2 hrs
Reference Values
The Access Immunoassay System calculates the test results automatically. A number in
pg/mL represents the amount of BNP present in the sample.
- BNP results less than or equal to 100 pg/mL are representative of normal values in patients without CHF.
- BNP results greater than 100 pg/mL are considered abnormal and suggestive of patients with CHF.
- BNP results of > 5000 pg/mL are considered very high values for BNP and exceed the upper limits of the BNP test.
Day(s) Test Set Up
Mon - Sun, continuously
CPT Code
83880