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Test Code LAB99 Lipase, Plasma or Serum

Reporting Title

Lipase

Methodology

Roche Diagnostics, Enzymatic - RMH, GMC, DH, DMH, GMH, WECC, PMH, GCMH, BH, OBH, MGH, MH, HMH, SH

Lipase, along with amylase, is useful in differential diagnosis of pancreatic disease. In acute pancreatitis, lipase rises within 4 to 8 hours, peaks after 24 hours, and decreases after 8 to 24 days. There is no correlation between lipase level and pancreatic damage.

 

VWH Lab Only:

LIP reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Enzyme Validator Set, is intended for the quantitative determination of Lipase activity in human serum or plasma in random access mode.

Performing Laboratory

OhioHealth Laboratory Services - RMH, GMC, DH, DMH, WECC, PMC, MGH, GMH, HMH, GCMH and OBH, MH, SH, BH and VWH Core Laboratories

Specimen Requirements

Collection Instructions:

1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Submit only 1 of the following specimens:

 

Preferred:

Serum

Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated.
Note:

1. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable..

2. Indicate serum on request form.

3. Label specimen appropriately (serum).

 

Alternate (Preferred for VWH Lab): 

Plasma

Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated.

Note: 

1. Indicate plasma on request form.

2. Label specimen appropriately (plasma).

VWH Lab:

3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells as soon as possible. A maximum limit of eight hours from the time of collection is recommended.

4. Separated serum or plasma should not remain at room temperature longer than 4 hours. If assays are not completed within 4 hours, serum or plasma should be stored at +2°C to +8°C. Refrigerated specimens are stable for 48 hrs. 

 

Roche Specimen Stability Information

Specimen Type Temperature Time
Serum or plasma Refrigerated 1 week
  Ambient  1 week
  Frozen  12 months

 

Specimen Transport Temperature

Refrigerate: OK

Ambient: OK

Frozen: OK

Reference Values

15-65 U/L

Critical values >200 U/L (emergency department only)

Alerting category: First instance called within 1 hour 

 

VWH Lab Only: 

22 – 51 U/L

 

Day(s) Test Set Up

Monday through Sunday; Continuously

Test Classification and CPT Coding

83690