Test Code LAB99 Lipase, Plasma or Serum
Reporting Title
Lipase
Methodology
Roche Diagnostics, Enzymatic - RMH, GMC, DH, DMH, GMH, WECC, PMH, GCMH, BH, OBH, MGH, MH, HMH, SH
Lipase, along with amylase, is useful in differential diagnosis of pancreatic disease. In acute pancreatitis, lipase rises within 4 to 8 hours, peaks after 24 hours, and decreases after 8 to 24 days. There is no correlation between lipase level and pancreatic damage.
VWH Lab Only:
LIP reagent, when used in conjunction with SYNCHRON LX® System(s), UniCel® DxC 860i System(s) and SYNCHRON® Systems Enzyme Validator Set, is intended for the quantitative determination of Lipase activity in human serum or plasma in random access mode.
Performing Laboratory
OhioHealth Laboratory Services - RMH, GMC, DH, DMH, WECC, PMC, MGH, GMH, HMH, GCMH and OBH, MH, SH, BH and VWH Core Laboratories
Specimen Requirements
Collection Instructions:
1. Serum and Plasma gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.
Submit only 1 of the following specimens:
Preferred:
Serum
Draw blood in a gold-top serum gel tube(s). Spin down and send 1 mL of serum refrigerated.
Note:
1. A speckled-top serum gel tube (s) or a plain, red-top tube(s) is also acceptable..
2. Indicate serum on request form.
3. Label specimen appropriately (serum).
Alternate (Preferred for VWH Lab):
Plasma
Draw blood in a mint green-top (heparin) gel tube(s). Spin down and send 1 mL of heparinized plasma refrigerated.
Note:
1. Indicate plasma on request form.
2. Label specimen appropriately (plasma).
VWH Lab:
3. Tubes of blood are to be kept closed at all times and in a vertical position. It is recommended that the serum or plasma be physically separated from contact with cells as soon as possible. A maximum limit of eight hours from the time of collection is recommended.
4. Separated serum or plasma should not remain at room temperature longer than 4 hours. If assays are not completed within 4 hours, serum or plasma should be stored at +2°C to +8°C. Refrigerated specimens are stable for 48 hrs.
Roche Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum or plasma | Refrigerated | 1 week |
Ambient | 1 week | |
Frozen | 12 months |
Specimen Transport Temperature
Refrigerate: OK
Ambient: OK
Frozen: OK
Reference Values
15-65 U/L
Critical values >200 U/L (emergency department only)
Alerting category: First instance called within 1 hour
VWH Lab Only:
22 – 51 U/L
Day(s) Test Set Up
Monday through Sunday; Continuously
Test Classification and CPT Coding
83690